Simple guide with unlock requirements + DLC included! Difficulties Hard Difficulty - defeat a boss on normal difficulty Lunar Difficulty - defeat a boss on hard difficulty Characters Characters can be unlocked by defeating specific bosses on hard mode or getting a certain amount of boss kills across all difficulties. Sniper - Beat the King's Arsenal on Hard difficulty or above (8 kills) SpellBlade - Beat the Scholar's Nest on Hard difficulty or above (20 kills) Bruiser - Beat the Red Darkhouse on Hard difficulty or above (30 kills) Defender - Beat the Churchmouse Streets on Hard difficulty or above (40 kills) Ancient - Beat the Emerald Lakeside on Hard difficulty or above (50 kills) -= DLC =- Hammer - Beat the Darkhouse Depths on Hard difficulty or above (16 Extra Boss) Pyro - Beat Atelier Aurum on Hard difficulty or above (28 Extra Boss) Gunner - Beat the Subterra Sanctum on Hard difficulty or above (50 Extra Boss) Shadow - Beat Extra Mode on Hard difficulty or above (70 Extra Boss) Item sets Sparkblade - Have a rabbit you control deal over 500 DPS in a fight (5 kills) Ruins - Have a rabbit you control gain 3 buffs at one time (12 kills) Timegem - Receive no rewards from a fight because you took too long to win (16 kills) Swiftflight - Pass through the Kingdom Outskirts in less than 2 minutes (25 kills) Darkbite - Beat any enemy in under 15 seconds (35 kills) Sacredflame - Deal over 2500 damage in a single hit (45 kills) Lakeshrine - Stand still doing nothing in the Emerald Lakeside for 2 full minutes (60 kills) Gladiator - Start a boss fight alone on 2 HP or less and win (70 kills) Youkai - Beat any enemy with exactly 77 gold on hand (80 kills) Haunted - Apply 4 debuffs to a single enemy at once (90 kills) -= DLC =- Glacier - Beat an enemy with your movement speed decreased by a significant amount (55 Extra Boss) Memory - Defeat an enemy only using your Special Cultist - Attempt to pet Saya in the shop 10 times Painters - Beat a run on any difficulty with all abilities upgraded with different gems Daynight - Defeat an enemy during enrage Sharpedge - Defeat an enemy having used your Primary and Secondary exactly 10 times each Oceans - Spend all of your money at a store Performers - Restart a run right after opening the first Treasuresphere Miners - Between your abilities and loot, have at least 7 things with a cooldown Teaparty - Buy at least 8 potions over the course of a run Music Outskirts - Pass through the Kingdom Outskirts on Lunar without taking damage. (100 kills) Nest - Defeat Twili, the Valedictorian Crow, while you are stopped in time. (120 kills) Arsenal - Defeat Merran, the Steeltooth Wolf, while time is slowed. (140 kills) Lighthouse - Defeat Ranalie, the Mythril Dragon, while she is dashing through the arena. (160 kills) Streets - Defeat Matti, the Paladin Mouse, while her guard is up. (180 boss kills) Lakeside - Defeat Avy, the Starry Frog, without standing still for longer than 5 seconds. (200 kills) Keep - Pass through the Pale Keep on Lunar without taking damage. (220 boss kills) Boss (Justice) - Win a run on Hard difficulty or above without a destination chosen at the start. (240 kills) Lakeside Vocals off - Win a run on Normal difficulty or above in less than 30 minutes. (250 kills???) Boss (Serious) - Win a run on Normal difficulty or above without opening treasure chests. (275 kills) Boss (Mysterious) - Win a run on Normal difficulty or above without talking to Asha, the Shopkeeper Cat. (300 kills) Pinnacle - Win a run on Normal difficulty or above without taking damage. (325 kills) Ending - Save Shira from the Spell, (350 kills) -= DLC =- Intro2 - Unlock Extra Mode Geode - Pass through the Crack in the Geode on Lunar without taking damage Depths - Defeat Farrah, the Witch's Hound, while standing in her "Deathwall" Sanct - On Normal difficulty or above, defeat every butterfly spawned by Eternal Bloom Aurum - On Normal difficulty or above, defeat Asha without anyone spending any gold previously in the run Darkhall - On Normal difficulty or above, defeat a Spelllock enemy in less than 10 seconds. BossHound - Collect 8 kinds of small creature trinkets BossOwl - Collect 5 kinds of floofball BossCat - Collect 50 trinkets Reflection - See your adventure through to its resolution Reflection2 - Beat a run on Hard on every rabbit SanctOffVocal - Unlock every item set Reflection2OffVocal - Win a run with every loot item at least once Closing statement DLC has released! Please put kill numbers in comments for new unlocks if applicable in comments, thanks! Thanks for all the information in the comments! comment info contributors: Alystaria Euclidean Giga Otomia JSlayerXero Kairose Lesserred Nyaner PhoenixWing pyxis Satsuiki Tsuruna(スネーク) wolfdragonrex 볼따구 Super Fan DLC extra boss kills The extra boss kill requirements are not fixed like base game list. Supposedly unlocking a tier by doing the challenge (not extra kills) will shift the requirements over. e.g. "A is 12, B is 24, C is 36." and if you got B by doing the challenge not the extra kills, then C and all following rewards would move up to the earlier tier. As if someone left the queue. Because of this it's not feasible to get community submitted extra boss kill requirements for the new rewards so unless there is new info contradicting the above they will not be updated.